Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration
Chung Joo-Ryun, 최종원, Fiorellini Joseph P., 황경균, 박창주,
소속 상세정보
( Chung Joo-Ryun ) - Hanyang University College of Medicine Department of Dentistry Division of Oral and Maxillofacial Surgery
최종원 ( Choi Jong-Won ) - Hanyang University College of Medicine Department of Dentistry Division of Oral and Maxillofacial Surgery
( Fiorellini Joseph P. ) - University of Pennsylvania School of Dental Medicine Department of Periodontics
황경균 ( Hwang Kyung-Gyun ) - Hanyang University College of Medicine Department of Dentistry Division of Oral and Maxillofacial Surgery
박창주 ( Park Chang-Joo ) - Hanyang University College of Medicine Department of Dentistry Division of Oral and Maxillofacial Surgery
Abstract
Background: For peripheral nerve regeneration, recent attentions have been paid to the nerve conduits made by tissue-engineering technique. Three major elements of tissue-engineering are cells, molecules, and scaffolds.
Methods: In this study, the attachments of nerve cells, including Schwann cells, on the nerve conduit and the effects of both growth factor and adhesion molecule on these attachments were investigated.
Results: The attachment of rapidly-proliferating cells, C6 cells and HS683 cells, on nerve conduit was better than that of slowly-proliferating cells, PC12 cells and Schwann cells, however, the treatment of nerve growth factor improved the attachment of slowly-proliferating cells. In addition, the attachment of Schwann cells on nerve conduit coated with fibronectin was as good as that of Schwann cells treated with glial cell line-derived neurotrophic factor (GDNF).
Conclusions: Growth factor changes nerve cell morphology and affects cell cycle time. And nerve growth factor or fibronectin treatment is indispensable for Schwann cell to be used for implantation in artificial nerve conduits.
키워드
Cellular Attachment; Fibronectins; Nerve Growth Factor; Nerve Cell; Tenascin
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